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Figure 2: Collection of scattered light by an integrating sphere. I
o
= incident light, I
s
= scattered light.
In order to perform useful measurements on scattering samples, it is necessary to
collect a high proportion of the scattered radiation. The integrating sphere is a highly
efficient collector of scattered radiation. Because of its design, the Diffuse
Reflectance accessory overcomes many of the problems associated with measuring
turbid or scattering samples, which include sloping baseline, poor signal-to-noise ratio
and high background absorbance.
Whilst the DRA should be used when measuring the transmittance of opaque or
diffusing solid samples, the accessory may also be used when measuring turbid
liquids.
Because the cuvette is located so as to be part of the wall of the sphere, a greater
proportion of the scattered radiation transmitted by the sample is collected by the
sphere, as shown in Figure 2 above
.
With the PTFE reference disk in position, a sample is placed in the cuvette, and light
is passed through the sample.
Factors affecting accuracy or precision
Below are the major factors that may affect the accuracy of measurements when using
the DRA.
Aperture area/total surface area ratio
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